Assembly Bundle

Title & Description

Enter a useful title for this assembly.

Cloning Methods

Which cloning methods would you like to include?

Backbone

Upload a .fasta file containing a single entry of your backbone sequence.

Insert

Is this a multi-sequence query?

  • If you did not select multi-sequence query, upload a .fasta file containing a single sequence entry of your full insert sequence.
  • If you selected multi-sequence query, upload a .fasta file containing multiple sequence entries that will compose your construct.

BLAST Query

Which BLAST databases would you like to query from to find parts?

Enter the minimum and maximum nucleotide sequence sizes to use that are returned from the database(s). BLAST alignment query results are filtered according to these sizes.

nt
nt

Enter the minimum and maximum nucleotide sequence sizes to use for synthesized assembly sequences.

nt
nt

Experiment

Enter experimental parameters you will be using for your assembly experiment in the lab. These values are important for primer desing and thermodynamic analysis.

mM
mM
mM
nM
℃

Nucleotides

Enter values for nucleotide costs of your assembly experiment.

$
$
$

PCR Enzyme

Please input: the total price for a tube of enzyme and the number of reactions per tube.

Solution Ranking

Enter values for how solutions are ranked based on your weight of considerations of number of parts and assembly cost.

PCR Probability

Enter the value for your expected experimental probabilities of success for PCR amplification of parts.

Psuccess