hhhhhhhhhhhhh Bundle

June 30, 2025, 6:38 p.m.

hhhh


These cost and time calculations exclude the cost and time of experimental steps that are common to all compared cloning methods.

Gibson
View Assembly


hhhhhhhhhhhhh Solution
A Gibson solution
  • 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
  • 60.72C Average Primer Tm 34nt Average Primer
  • 93.00% BLAST nt 7.00% Synthetic nt

Golden Gate
View Assembly


hhhhhhhhhhhhh Solution
A Golden Gate solution
  • 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 707nt Longest Part 111nt Shortest Part
  • 60.62C Average Primer Tm 31nt Average Primer Restriction Enzymes
  • 94.00% BLAST nt 6.00% Synthetic nt

PCR
View Assembly


hhhhhhhhhhhhh Solution
A PCR solution
  • 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
  • 60.72C Average Primer Tm 34nt Average Primer
  • 93.00% BLAST nt 7.00% Synthetic nt