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June 30, 2025, 6:38 p.m.
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These cost and time calculations exclude the cost and time of experimental steps that are common to all compared cloning methods.
Gibson
View Assembly
hhhhhhhhhhhhh Solution
A Gibson solution
- 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
- 60.72C Average Primer Tm 34nt Average Primer
- 93.00% BLAST nt 7.00% Synthetic nt
Golden Gate
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hhhhhhhhhhhhh Solution
A Golden Gate solution
- 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 707nt Longest Part 111nt Shortest Part
- 60.62C Average Primer Tm 31nt Average Primer Restriction Enzymes
- 94.00% BLAST nt 6.00% Synthetic nt
PCR
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hhhhhhhhhhhhh Solution
A PCR solution
- 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
- 60.72C Average Primer Tm 34nt Average Primer
- 93.00% BLAST nt 7.00% Synthetic nt