CS2 test Bundle

June 29, 2025, 10:55 p.m.

publication test for bundle


These cost and time calculations exclude the cost and time of experimental steps that are common to all compared cloning methods.

Gibson
View Assembly


CS2 test Solution
A Gibson solution
  • 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
  • 60.72C Average Primer Tm 34nt Average Primer
  • 89.00% BLAST nt 11.00% Synthetic nt

Golden Gate
View Assembly


CS2 test Solution
A Golden Gate solution
  • 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
  • 60.72C Average Primer Tm 30nt Average Primer Restriction Enzymes
  • 89.00% BLAST nt 11.00% Synthetic nt

PCR
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CS2 test Solution
A PCR solution
  • 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
  • 60.72C Average Primer Tm 34nt Average Primer
  • 89.00% BLAST nt 11.00% Synthetic nt