CS2 test Gibson

June 29, 2025

This is an overview of your assembly. Under the Solutions section you will find solutions that Smithy has created for you. Click on View Solution to see the parts (purchasable DNA sequences), primer designs for each part, and more that Smithy has created for that unique assembly solution.

The cost and time calculations exclude the cost and time of experimental steps that are common to all compared cloning methods.

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General Info

  • Method: Gibson
  • Overlap: 30nt

BLAST Query

  • AddGene iGEM DNASU
  • Min BLAST seq size: 50nt
  • Max BLAST seq size: 5000nt
  • Min synthetic seq size: 50nt
  • Max synthetic seq size: 5000nt

Experiment

  • Monovalent ion concentration: 50.0mM
  • Divalent ion concentration: 1.5mM
  • dNTP concentration: 0.8mM
  • DNA concentration: 50.0nM
  • Melting temperature: 60.0C

Solution(s)

CS2 test Solution View Solution

June 29, 2025, 10:55 p.m.
  • 4 Parts 8 Primers
  • 60.72C Average Primer Tm 34nt Average Primer
  • 89.00% BLAST nt 11.00% Synthetic nt