lac-mcherry-theo-gfp Gibson

December 14, 2024

This is an overview of your assembly. Under the Solutions section you will find solutions that Smithy has created for you. Click on View Solution to see the parts (purchasable DNA sequences), primer designs for each part, and more that Smithy has created for that unique assembly solution.

The cost and time calculations exclude the cost and time of experimental steps that are common to all compared cloning methods.

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General Info

  • Method: Gibson
  • Overlap: 30nt

BLAST Query

  • AddGene
  • Min BLAST seq size: 100nt
  • Max BLAST seq size: 3000nt
  • Min synthetic seq size: 100nt
  • Max synthetic seq size: 3000nt

Experiment

  • Monovalent ion concentration: 50.0mM
  • Divalent ion concentration: 1.5mM
  • dNTP concentration: 0.8mM
  • DNA concentration: 50.0nM
  • Melting temperature: 60.0C

Solution(s)

lac-mcherry-theo-gfp Solution View Solution

Dec. 14, 2024, 11:31 p.m.
  • 4 Parts 8 Primers
  • 60.23C Average Primer Tm 32nt Average Primer
  • 97.00% BLAST nt 3.00% Synthetic nt