lac-mcherry-theo-gfp Solution Gibson
Dec. 14, 2024, 11:31 p.m.
- 4 Parts 8 Primers
- 60.23C Average Primer Tm 32nt Average Primer
- 97.00% BLAST nt 3.00% Synthetic nt 4 BLAST Parts 0 Synthetic Parts 717nt Longest Part 57nt Shortest Part
Parts Download Parts
Below is a summary table of the DNA sequences that make up this assembly solution. Further details on each part can be viewed by clicking View Part for each listed below this table.
Name | Database | Length | Extended Length | Query Start | Query End | Subject Start | Subject End | Position |
---|---|---|---|---|---|---|---|---|
115767-addgene | addgene | 133 | 163 | 0 | 133 | 4272 | 4140 | 0 |
89463-addgene | addgene | 711 | 741 | 133 | 844 | 5445 | 4735 | 1 |
53116-addgene | addgene | 57 | 87 | 844 | 901 | 6258 | 6314 | 2 |
82368-addgene | addgene | 717 | 747 | 901 | 1618 | 211 | 927 | 3 |
backbone | NONE | 2151 | 2181 | 1619 | 3769 | 0 | 0 | 4 |
Plasmid Map

Parts List
115767-addgene addgene View Part
163nt Extended 133nt Template
- Assembly Sequence: GTACGGAGCGAGACCgtgagctgat...ctggcacgacatggtgagcaagggc
- Template Sequence: gtgagctgataccgctcgccgcagc...gattcattaatgcagctggcacgac
89463-addgene addgene View Part
741nt Extended 711nt Template
- Assembly Sequence: tgcagctggcacgacatggtgagca...gtacaagtaaggtgataccagcatc
- Template Sequence: atggtgagcaagggcgaggaggata...cggcatggacgagctgtacaagtaa
53116-addgene addgene View Part
87nt Extended 57nt Template
- Assembly Sequence: gagctgtacaagtaaggtgatacca...taacaacaagatgagtaaaggagaa
- Template Sequence: ggtgataccagcatcgtcttgatgc...agcaccctgctaaggtaacaacaag
82368-addgene addgene View Part
747nt Extended 717nt Template
- Assembly Sequence: taaggtaacaacaagatgagtaaag...atacaaataaGGTCTCACCATTCCT
- Template Sequence: atgagtaaaggagaagaacttttca...tggcatggatgaactatacaaataa
backbone View Part
2181nt Extended 2151nt Template
- Assembly Sequence: gaactatacaaataaGGTCTCACCA...GAGCGAGACCgtgagctgataccgc
- Template Sequence: GGTCTCACCATTCCTGTAGACTTCT...CGAAGTCTTGGTACGGAGCGAGACC
Primers Download Primers
Below is a summary table of the primers needed for each part of this assembly solution (shown above). Further details on each primer can be viewed by clicking View Primer for each listed below this table.
Name | Type | Tm (C) | GC% | Hairpin | hp Tm (C) | hp ΔG | Homodimer | hd Tm (C) | hd ΔG |
---|---|---|---|---|---|---|---|---|---|
115767-addgene forward primer | fwd | 60.471 | 29.03 | True | 56.568 | -2041.590 | True | 23.361 | -6542.435 |
115767-addgene reverse primer | rvs | 61.684 | 65.52 | True | 35.243 | 230.700 | True | 8.420 | -5998.441 |
89463-addgene forward primer | fwd | 62.012 | 63.33 | True | 50.295 | -822.084 | True | 16.903 | -7044.691 |
89463-addgene reverse primer | rvs | 61.920 | 50.0 | True | 39.281 | -148.213 | True | -0.026 | -4952.691 |
53116-addgene forward primer | fwd | 59.473 | 48.39 | True | 41.613 | -271.143 | True | -3.445 | -4572.426 |
53116-addgene reverse primer | rvs | 58.734 | 38.24 | False | 0.000 | 0.000 | True | -33.069 | -2810.310 |
82368-addgene forward primer | fwd | 60.173 | 31.58 | True | 37.008 | -1.130 | True | -20.930 | -3085.011 |
82368-addgene reverse primer | rvs | 60.214 | 18.42 | True | 39.281 | -148.213 | True | -25.949 | -3041.586 |
backbone forward primer | fwd | 58.913 | 9.68 | True | 41.613 | -271.143 | True | -6.788 | -2470.064 |
backbone reverse primer | rvs | 58.674 | 32.14 | True | 57.968 | -2001.044 | True | 11.075 | -3499.185 |
Primers List
115767-addgene forward primer View Primer
fwd 60.471°C Hairpin Homodimer
- Sequence: GTACGGAGCGAGACCgtgagctgataccgct
115767-addgene reverse primer View Primer
rvs 61.684°C Hairpin Homodimer
- Sequence: gcccttgctcaccatgtcgtgccagctgc
89463-addgene forward primer View Primer
fwd 62.012°C Hairpin Homodimer
- Sequence: tgcagctggcacgacatggtgagcaagggc
89463-addgene reverse primer View Primer
rvs 61.920°C Hairpin Homodimer
- Sequence: gatgctggtatcaccttacttgtacagctcgtcc
53116-addgene forward primer View Primer
fwd 59.473°C Hairpin Homodimer
- Sequence: gagctgtacaagtaaggtgataccagcatcg
53116-addgene reverse primer View Primer
rvs 58.734°C Homodimer
- Sequence: ttctcctttactcatcttgttgttaccttagcag
82368-addgene forward primer View Primer
fwd 60.173°C Hairpin Homodimer
- Sequence: taaggtaacaacaagatgagtaaaggagaagaactttt
82368-addgene reverse primer View Primer
rvs 60.214°C Hairpin Homodimer
- Sequence: AGGAATGGTGAGACCttatttgtatagttcatccatgc
backbone forward primer View Primer
fwd 58.913°C Hairpin Homodimer
- Sequence: gaactatacaaataaGGTCTCACCATTCCTG
backbone reverse primer View Primer
rvs 58.674°C Hairpin Homodimer
- Sequence: gcggtatcagctcacGGTCTCGCTCCGT