lac-mcherry-theo-gfp Solution Gibson

Dec. 14, 2024, 11:31 p.m.

  • 4 Parts 8 Primers
  • 60.23C Average Primer Tm 32nt Average Primer
  • 97.00% BLAST nt 3.00% Synthetic nt 4 BLAST Parts 0 Synthetic Parts 717nt Longest Part 57nt Shortest Part
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Parts Download Parts

Below is a summary table of the DNA sequences that make up this assembly solution. Further details on each part can be viewed by clicking View Part for each listed below this table.

Query Start/End: The start/end index of the part on the insert sequence. Defaults to 0.
Subject Start/End: The start/end index of the part on its original sequence. Defaults to 0.
Position: The location of the part in the assembly solution.
Name Database Length Extended Length Query Start Query End Subject Start Subject End Position
115767-addgene addgene 133 163 0 133 4272 4140 0
89463-addgene addgene 711 741 133 844 5445 4735 1
53116-addgene addgene 57 87 844 901 6258 6314 2
82368-addgene addgene 717 747 901 1618 211 927 3
backbone NONE 2151 2181 1619 3769 0 0 4

Plasmid Map

Parts List

115767-addgene addgene View Part

163nt Extended 133nt Template

  • Assembly Sequence: GTACGGAGCGAGACCgtgagctgat...ctggcacgacatggtgagcaagggc
  • Template Sequence: gtgagctgataccgctcgccgcagc...gattcattaatgcagctggcacgac

89463-addgene addgene View Part

741nt Extended 711nt Template

  • Assembly Sequence: tgcagctggcacgacatggtgagca...gtacaagtaaggtgataccagcatc
  • Template Sequence: atggtgagcaagggcgaggaggata...cggcatggacgagctgtacaagtaa

53116-addgene addgene View Part

87nt Extended 57nt Template

  • Assembly Sequence: gagctgtacaagtaaggtgatacca...taacaacaagatgagtaaaggagaa
  • Template Sequence: ggtgataccagcatcgtcttgatgc...agcaccctgctaaggtaacaacaag

82368-addgene addgene View Part

747nt Extended 717nt Template

  • Assembly Sequence: taaggtaacaacaagatgagtaaag...atacaaataaGGTCTCACCATTCCT
  • Template Sequence: atgagtaaaggagaagaacttttca...tggcatggatgaactatacaaataa

backbone View Part

2181nt Extended 2151nt Template

  • Assembly Sequence: gaactatacaaataaGGTCTCACCA...GAGCGAGACCgtgagctgataccgc
  • Template Sequence: GGTCTCACCATTCCTGTAGACTTCT...CGAAGTCTTGGTACGGAGCGAGACC

Primers Download Primers

Below is a summary table of the primers needed for each part of this assembly solution (shown above). Further details on each primer can be viewed by clicking View Primer for each listed below this table.

hp Tm (C): The melting temperature of the primer's hairpin (hp) structure.
hp ΔG: The Gibbs free energy of the primer's hairpin (hp) structure.
hd Tm (C): The melting temperature of the primer's homodimer (hd) structure.
hd ΔG: The Gibbs free energy of the primer's homodimer (hd) structure.
Name Type Tm (C) GC% Hairpin hp Tm (C) hp ΔG Homodimer hd Tm (C) hd ΔG
115767-addgene forward primer fwd 60.471 29.03 True 56.568 -2041.590 True 23.361 -6542.435
115767-addgene reverse primer rvs 61.684 65.52 True 35.243 230.700 True 8.420 -5998.441
89463-addgene forward primer fwd 62.012 63.33 True 50.295 -822.084 True 16.903 -7044.691
89463-addgene reverse primer rvs 61.920 50.0 True 39.281 -148.213 True -0.026 -4952.691
53116-addgene forward primer fwd 59.473 48.39 True 41.613 -271.143 True -3.445 -4572.426
53116-addgene reverse primer rvs 58.734 38.24 False 0.000 0.000 True -33.069 -2810.310
82368-addgene forward primer fwd 60.173 31.58 True 37.008 -1.130 True -20.930 -3085.011
82368-addgene reverse primer rvs 60.214 18.42 True 39.281 -148.213 True -25.949 -3041.586
backbone forward primer fwd 58.913 9.68 True 41.613 -271.143 True -6.788 -2470.064
backbone reverse primer rvs 58.674 32.14 True 57.968 -2001.044 True 11.075 -3499.185

Primers List

115767-addgene forward primer View Primer

fwd 60.471°C Hairpin Homodimer

  • Sequence: GTACGGAGCGAGACCgtgagctgataccgct

115767-addgene reverse primer View Primer

rvs 61.684°C Hairpin Homodimer

  • Sequence: gcccttgctcaccatgtcgtgccagctgc

89463-addgene forward primer View Primer

fwd 62.012°C Hairpin Homodimer

  • Sequence: tgcagctggcacgacatggtgagcaagggc

89463-addgene reverse primer View Primer

rvs 61.920°C Hairpin Homodimer

  • Sequence: gatgctggtatcaccttacttgtacagctcgtcc

53116-addgene forward primer View Primer

fwd 59.473°C Hairpin Homodimer

  • Sequence: gagctgtacaagtaaggtgataccagcatcg

53116-addgene reverse primer View Primer

rvs 58.734°C Homodimer

  • Sequence: ttctcctttactcatcttgttgttaccttagcag

82368-addgene forward primer View Primer

fwd 60.173°C Hairpin Homodimer

  • Sequence: taaggtaacaacaagatgagtaaaggagaagaactttt

82368-addgene reverse primer View Primer

rvs 60.214°C Hairpin Homodimer

  • Sequence: AGGAATGGTGAGACCttatttgtatagttcatccatgc

backbone forward primer View Primer

fwd 58.913°C Hairpin Homodimer

  • Sequence: gaactatacaaataaGGTCTCACCATTCCTG

backbone reverse primer View Primer

rvs 58.674°C Hairpin Homodimer

  • Sequence: gcggtatcagctcacGGTCTCGCTCCGT
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