cs2 test with all db Golden Gate

June 29, 2025

This is an overview of your assembly. Under the Solutions section you will find solutions that Smithy has created for you. Click on View Solution to see the parts (purchasable DNA sequences), primer designs for each part, and more that Smithy has created for that unique assembly solution.

The cost and time calculations exclude the cost and time of experimental steps that are common to all compared cloning methods.

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General Info

  • Method: Golden Gate
  • Overhangs: 20 overhangs - 98.1% fidelity

BLAST Query

  • AddGene iGEM DNASU
  • Min BLAST seq size: 100nt
  • Max BLAST seq size: 5000nt
  • Min synthetic seq size: 100nt
  • Max synthetic seq size: 5000nt

Experiment

  • Monovalent ion concentration: 50.0mM
  • Divalent ion concentration: 1.5mM
  • dNTP concentration: 0.8mM
  • DNA concentration: 50.0nM
  • Melting temperature: 60.0C

Solutions

cs2 test with all db Solution View Solution

June 29, 2025, 9:36 p.m.
  • 4 Parts 8 Primers
  • 60.72C Average Primer Tm 30nt Average Primer
  • 88.00% BLAST nt 12.00% Synthetic nt