cs2 test with db Solution Golden Gate
June 29, 2025, 9:30 p.m.
- 4 Parts 8 Primers
- 60.88C Average Primer Tm 30nt Average Primer 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
- 80.00% BLAST nt 20.00% Synthetic nt
Parts Download Parts
Below is a summary table of the DNA sequences that make up this assembly solution. Further details on each part can be viewed by clicking View Part for each listed below this table.
Name | Database | Length | Extended Length | Query Start | Query End | Subject Start | Subject End | Position |
---|---|---|---|---|---|---|---|---|
120427-addgene | addgene | 133 | 155 | 4 | 137 | 18561 | 18429 | 0 |
79124.2-addgene | addgene | 711 | 733 | 141 | 852 | 810 | 100 | 1 |
Synthetic-1.112 | NONE | 112 | 134 | 856 | 968 | 0 | 0 | 2 |
79550-addgene | addgene | 717 | 739 | 972 | 1689 | 1132 | 1848 | 3 |
pBR322_b | NONE | 4361 | 4383 | 1694 | 6054 | 0 | 0 | 4 |
Plasmid Map

Parts List
120427-addgene addgene View Part
155nt Extended 133nt Template
- Assembly Sequence: GGTCTCnAGTGgtgagctgataccg...gcagctggcacgacCAGGnGAGACC
- Template Sequence: gtgagctgataccgctcgccgcagc...gattcattaatgcagctggcacgac
79124.2-addgene addgene View Part
733nt Extended 711nt Template
- Assembly Sequence: GGTCTCnCAGGatggtgagcaaggg...agctgtacaagtaaACTCnGAGACC
- Template Sequence: atggtgagcaagggcgaggaggata...cggcatggacgagctgtacaagtaa
Synthetic-1.112 View Part
134nt Extended 112nt Template
- Assembly Sequence: GGTCTCnACTCggtgataccagcat...aattcttgttgaatAAAAnGAGACC
- Template Sequence: ggtgataccagcatcgtcttgatgc...ggagttgtcccaattcttgttgaat
79550-addgene addgene View Part
739nt Extended 717nt Template Internal Cutsite(s) - Adjust probability of success for future experiments
- Assembly Sequence: GGTCTCnAAAAatgagtaaaggaga...atctctacaaataaAGACnGAGACC
- Template Sequence: atgagtaaaggagaagaacttttca...tggcatggatgatctctacaaataa
pBR322_b View Part
4383nt Extended 4361nt Template Internal Cutsite(s) - Adjust probability of success for future experiments
- Assembly Sequence: GGTCTCnAGACCTTATCATCGATAA...CTCATGTTTGACAGAGTGnGAGACC
- Template Sequence: CTTATCATCGATAAGCTTTAATGCG...CTTCAAGAATTCTCATGTTTGACAG
Primers Download Primers
Below is a summary table of the primers needed for each part of this assembly solution (shown above). Further details on each primer can be viewed by clicking View Primer for each listed below this table.
Name | Type | Tm (C) | GC% | Hairpin | hp Tm (C) | hp ΔG | Homodimer | hd Tm (C) | hd ΔG |
---|---|---|---|---|---|---|---|---|---|
120427-addgene forward primer | fwd | 60.471 | 33.33 | True | 48.792 | -1260.012 | True | 16.964 | -4571.702 |
120427-addgene reverse primer | rvs | 61.684 | 40.0 | True | 57.817 | -2144.785 | True | 18.461 | -5188.669 |
79124.2-addgene forward primer | fwd | 62.012 | 34.62 | True | 41.611 | -254.908 | True | -24.870 | -2693.161 |
79124.2-addgene reverse primer | rvs | 61.920 | 30.0 | False | 0.000 | 0.000 | True | -8.830 | -3413.286 |
Synthetic-1.112 forward primer | fwd | 59.473 | 33.33 | True | 38.109 | -59.863 | True | -30.105 | -3125.981 |
Synthetic-1.112 reverse primer | rvs | 60.130 | 22.58 | True | 53.877 | -1047.648 | True | -23.066 | -2929.371 |
79550-addgene forward primer | fwd | 60.173 | 20.59 | True | 37.663 | -70.162 | True | -20.930 | -3085.011 |
79550-addgene reverse primer | rvs | 61.240 | 23.53 | False | 0.000 | 0.000 | True | -9.787 | -3647.801 |
pBR322_b forward primer | fwd | 60.806 | 0.0 | True | 55.376 | -2002.494 | True | 14.497 | -5348.232 |
pBR322_b reverse primer | rvs | 60.872 | 0.0 | True | 33.558 | 399.571 | True | 9.560 | -5625.021 |
Primers List
120427-addgene forward primer View Primer
fwd 60.471°C Hairpin Homodimer
- Sequence: GGTCTCnAGTGgtgagctgataccgct
120427-addgene reverse primer View Primer
rvs 61.684°C Hairpin Homodimer
- Sequence: GGTCTCnCCTGgtcgtgccagctgc
79124.2-addgene forward primer View Primer
fwd 62.012°C Hairpin Homodimer
- Sequence: GGTCTCnCAGGatggtgagcaagggc
79124.2-addgene reverse primer View Primer
rvs 61.920°C Homodimer
- Sequence: GGTCTCnGAGTttacttgtacagctcgtcc
Synthetic-1.112 forward primer View Primer
fwd 59.473°C Hairpin Homodimer
- Sequence: GGTCTCnACTCggtgataccagcatcg
Synthetic-1.112 reverse primer View Primer
rvs 60.130°C Hairpin Homodimer
- Sequence: GGTCTCnTTTTattcaacaagaattgggaca
79550-addgene forward primer View Primer
fwd 60.173°C Hairpin Homodimer
- Sequence: GGTCTCnAAAAatgagtaaaggagaagaactttt
79550-addgene reverse primer View Primer
rvs 61.240°C Homodimer
- Sequence: GGTCTCnGTCTttatttgtagagatcatccatgc
pBR322_b forward primer View Primer
fwd 60.806°C Hairpin Homodimer
- Sequence: GGTCTCnAGACCTTATCATCGATAAGCTTTAATGC
pBR322_b reverse primer View Primer
rvs 60.872°C Hairpin Homodimer
- Sequence: GGTCTCnCACTCTGTCAAACATGAGAATTCTTGAA