ggggggggg Solution PCR

June 30, 2025, 4:56 p.m.

  • 4 Parts 8 Primers
  • 60.78C Average Primer Tm 34nt Average Primer 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
  • 80.00% BLAST nt 20.00% Synthetic nt
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Parts Download Parts

Below is a summary table of the DNA sequences that make up this assembly solution. Further details on each part can be viewed by clicking View Part for each listed below this table.

Query Start/End: The start/end index of the part on the insert sequence. Defaults to 0.
Subject Start/End: The start/end index of the part on its original sequence. Defaults to 0.
Position: The location of the part in the assembly solution.
Name Database Length Extended Length Query Start Query End Subject Start Subject End Position
118302-addgene addgene 133 163 0 133 4584 4452 0
79419-addgene addgene 711 741 133 844 5403 6113 1
Synthetic-1.112 NONE 112 142 844 956 0 0 2
102673-addgene addgene 717 747 956 1673 104 820 3
pBR322_b NONE 4361 4391 1674 6034 0 0 4

Plasmid Map

Parts List

118302-addgene addgene View Part

163nt Extended 133nt Template

  • Assembly Sequence: TCTCATGTTTGACAGgtgagctgat...ctggcacgacatggtgagcaagggc
  • Template Sequence: gtgagctgataccgctcgccgcagc...gattcattaatgcagctggcacgac

79419-addgene addgene View Part

741nt Extended 711nt Template

  • Assembly Sequence: tgcagctggcacgacatggtgagca...gtacaagtaaggtgataccagcatc
  • Template Sequence: atggtgagcaagggcgaggaggata...cggcatggacgagctgtacaagtaa

Synthetic-1.112 View Part

142nt Extended 112nt Template

  • Assembly Sequence: gagctgtacaagtaaggtgatacca...cttgttgaatatgagtaaaggagaa
  • Template Sequence: ggtgataccagcatcgtcttgatgc...ggagttgtcccaattcttgttgaat

102673-addgene addgene View Part

747nt Extended 717nt Template

  • Assembly Sequence: caattcttgttgaatatgagtaaag...atacaaataaCTTATCATCGATAAG
  • Template Sequence: atgagtaaaggagaagaacttttca...tggcatggatgaactatacaaataa

pBR322_b View Part

4391nt Extended 4361nt Template

  • Assembly Sequence: gaactatacaaataaCTTATCATCG...TGTTTGACAGgtgagctgataccgc
  • Template Sequence: CTTATCATCGATAAGCTTTAATGCG...CTTCAAGAATTCTCATGTTTGACAG

Primers Download Primers

Below is a summary table of the primers needed for each part of this assembly solution (shown above). Further details on each primer can be viewed by clicking View Primer for each listed below this table.

hp Tm (C): The melting temperature of the primer's hairpin (hp) structure.
hp ΔG: The Gibbs free energy of the primer's hairpin (hp) structure.
hd Tm (C): The melting temperature of the primer's homodimer (hd) structure.
hd ΔG: The Gibbs free energy of the primer's homodimer (hd) structure.
Name Type Tm (C) GC% Hairpin hp Tm (C) hp ΔG Homodimer hd Tm (C) hd ΔG
118302-addgene forward primer fwd 60.471 29.03 True 41.615 -385.626 True 19.277 -7324.651
118302-addgene reverse primer rvs 61.684 65.52 True 35.243 230.700 True 8.420 -5998.441
79419-addgene forward primer fwd 62.012 63.33 True 50.295 -822.084 True 16.903 -7044.691
79419-addgene reverse primer rvs 61.920 50.0 True 39.281 -148.213 True -0.026 -4952.691
Synthetic-1.112 forward primer fwd 59.473 48.39 True 41.613 -271.143 True -3.445 -4572.426
Synthetic-1.112 reverse primer rvs 60.130 34.29 True 53.877 -1047.648 True -23.066 -2929.371
102673-addgene forward primer fwd 60.173 28.95 True 43.634 -565.455 True -24.427 -3386.826
102673-addgene reverse primer rvs 60.214 18.42 True 43.226 -840.354 True 8.307 -5731.151
pBR322_b forward primer fwd 60.806 7.69 True 48.641 -1501.254 True 18.725 -5292.685
pBR322_b reverse primer rvs 60.872 23.08 True 36.900 11.873 True 9.560 -5625.021

Primers List

118302-addgene forward primer View Primer

fwd 60.471°C Hairpin Homodimer

  • Sequence: TCTCATGTTTGACAGgtgagctgataccgct

118302-addgene reverse primer View Primer

rvs 61.684°C Hairpin Homodimer

  • Sequence: gcccttgctcaccatgtcgtgccagctgc

79419-addgene forward primer View Primer

fwd 62.012°C Hairpin Homodimer

  • Sequence: tgcagctggcacgacatggtgagcaagggc

79419-addgene reverse primer View Primer

rvs 61.920°C Hairpin Homodimer

  • Sequence: gatgctggtatcaccttacttgtacagctcgtcc

Synthetic-1.112 forward primer View Primer

fwd 59.473°C Hairpin Homodimer

  • Sequence: gagctgtacaagtaaggtgataccagcatcg

Synthetic-1.112 reverse primer View Primer

rvs 60.130°C Hairpin Homodimer

  • Sequence: ttctcctttactcatattcaacaagaattgggaca

102673-addgene forward primer View Primer

fwd 60.173°C Hairpin Homodimer

  • Sequence: caattcttgttgaatatgagtaaaggagaagaactttt

102673-addgene reverse primer View Primer

rvs 60.214°C Hairpin Homodimer

  • Sequence: CTTATCGATGATAAGttatttgtatagttcatccatgc

pBR322_b forward primer View Primer

fwd 60.806°C Hairpin Homodimer

  • Sequence: gaactatacaaataaCTTATCATCGATAAGCTTTAATGC

pBR322_b reverse primer View Primer

rvs 60.872°C Hairpin Homodimer

  • Sequence: gcggtatcagctcacCTGTCAAACATGAGAATTCTTGAA
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