CS2 15-nt Tail Solution Golden Gate

July 24, 2025, 6:38 p.m.

  • 4 Parts 8 Primers
  • 60.52C Average Primer Tm 33nt Average Primer 3 BLAST Parts 1 Synthetic Parts 700nt Longest Part 111nt Shortest Part
  • 81.00% BLAST nt 19.00% Synthetic nt
Download Parts Download Primers Download Order

Parts Download Parts

Below is a summary table of the DNA sequences that make up this assembly solution. Further details on each part can be viewed by clicking View Part for each listed below this table.

Query Start/End: The start/end index of the part on the insert sequence. Defaults to 0.
Subject Start/End: The start/end index of the part on its original sequence. Defaults to 0.
Position: The location of the part in the assembly solution.
Name Database Length Extended Length Query Start Query End Subject Start Subject End Position
120397-addgene addgene 111 125 4 115 8436 8304 0
46918.2-addgene addgene 685 723 119 804 124 834 1
Synthetic-1.112 NONE 112 167 808 920 0 0 2
73974-addgene addgene 700 714 924 1624 19309 20025 3
pBR322_b NONE 4361 4395 1662 6022 0 0 4

Plasmid Map

Parts List

120397-addgene addgene View Part

125nt Extended 111nt Template

  • Assembly Sequence: GGTCTCNgagctgataccgctcgcc...ccgcgcgttggccgattcNGAGACC
  • Template Sequence: gagctgataccgctcgccgcagccg...cctctccccgcgcgttggccgattc

46918.2-addgene addgene View Part

723nt Extended 685nt Template

  • Assembly Sequence: GGTCTCNattcattaatgcagctgg...agggccgccactccaccgNGAGACC
  • Template Sequence: atggtgagcaagggcgaggaggata...cgcgccgagggccgccactccaccg

Synthetic-1.112 View Part

167nt Extended 112nt Template

  • Assembly Sequence: GGTCTCNaccggcggcatggacgag...gttgaatatgagtaaaggNGAGACC
  • Template Sequence: ggtgataccagcatcgtcttgatgc...ggagttgtcccaattcttgttgaat

73974-addgene addgene View Part

714nt Extended 700nt Template Internal Cutsite(s) - Adjust probability of success for future experiments

  • Assembly Sequence: GGTCTCNaaggagaagaacttttca...acatggcatggatgaactNGAGACC
  • Template Sequence: aaggagaagaacttttcactggagt...ggattacacatggcatggatgaact

pBR322_b View Part

4395nt Extended 4361nt Template Internal Cutsite(s) - Adjust probability of success for future experiments

  • Assembly Sequence: GGTCTCNaactatacaaataaCTTA...CATGTTTGACAGgtgagcNGAGACC
  • Template Sequence: CTTATCATCGATAAGCTTTAATGCG...CTTCAAGAATTCTCATGTTTGACAG

Primers Download Primers

Below is a summary table of the primers needed for each part of this assembly solution (shown above). Further details on each primer can be viewed by clicking View Primer for each listed below this table.

hp Tm (C): The melting temperature of the primer's hairpin (hp) structure.
hp ΔG: The Gibbs free energy of the primer's hairpin (hp) structure.
hd Tm (C): The melting temperature of the primer's homodimer (hd) structure.
hd ΔG: The Gibbs free energy of the primer's homodimer (hd) structure.
Name Type Tm (C) GC% Hairpin hp Tm (C) hp ΔG Homodimer hd Tm (C) hd ΔG
120397-addgene forward primer fwd 61.237 43.48 True 43.375 -598.165 True -28.184 -4244.021
120397-addgene reverse primer rvs 59.840 42.86 True 43.699 -424.964 True 2.079 -5455.321
46918.2-addgene forward primer fwd 62.012 43.48 True 58.993 -1443.514 True 16.903 -7044.691
46918.2-addgene reverse primer rvs 61.855 50.0 False 0.000 0.000 True -41.601 -1676.980
Synthetic-1.112 forward primer fwd 59.473 49.06 True 46.441 -602.665 True 28.483 -9464.991
Synthetic-1.112 reverse primer rvs 60.130 28.95 True 53.877 -1047.648 True -23.066 -2929.371
73974-addgene forward primer fwd 59.777 28.57 True 40.204 -244.399 True 6.990 -2238.472
73974-addgene reverse primer rvs 59.197 33.33 False 0.000 0.000 True -5.037 -3407.721
pBR322_b forward primer fwd 60.806 4.44 True 48.641 -1501.254 True 18.725 -5292.685
pBR322_b reverse primer rvs 60.872 10.81 True 36.900 11.873 True 9.560 -5625.021

Primers List

120397-addgene forward primer View Primer

fwd 61.237°C Hairpin Homodimer

  • Sequence: GGTCTCNgagctgataccgctcg

120397-addgene reverse primer View Primer

rvs 59.840°C Hairpin Homodimer

  • Sequence: GGTCTCNgaatcggccaacgc

46918.2-addgene forward primer View Primer

fwd 62.012°C Hairpin Homodimer

  • Sequence: GGTCTCNattcattaatgcagctggcacgacatggtgagcaagggc

46918.2-addgene reverse primer View Primer

rvs 61.855°C Homodimer

  • Sequence: GGTCTCNcggtggagtggcg

Synthetic-1.112 forward primer View Primer

fwd 59.473°C Hairpin Homodimer

  • Sequence: GGTCTCNaccggcggcatggacgagctgtacaagtaaggtgataccagcatcg

Synthetic-1.112 reverse primer View Primer

rvs 60.130°C Hairpin Homodimer

  • Sequence: GGTCTCNcctttactcatattcaacaagaattgggaca

73974-addgene forward primer View Primer

fwd 59.777°C Hairpin Homodimer

  • Sequence: GGTCTCNaaggagaagaacttttcactg

73974-addgene reverse primer View Primer

rvs 59.197°C Homodimer

  • Sequence: GGTCTCNagttcatccatgccatg

pBR322_b forward primer View Primer

fwd 60.806°C Hairpin Homodimer

  • Sequence: GGTCTCNaactatacaaataaCTTATCATCGATAAGCTTTAATGC

pBR322_b reverse primer View Primer

rvs 60.872°C Hairpin Homodimer

  • Sequence: GGTCTCNgctcacCTGTCAAACATGAGAATTCTTGAA
Download Parts Download Primers