CS2 Solution Golden Gate

Aug. 14, 2025, 4:13 p.m.

  • 4 Parts 8 Primers
  • 60.59C Average Primer Tm 32nt Average Primer 4 BLAST Parts 0 Synthetic Parts 707nt Longest Part 35nt Shortest Part
  • 98.00% BLAST nt 2.00% Synthetic nt
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Parts Download Parts

Below is a summary table of the DNA sequences that make up this assembly solution. Further details on each part can be viewed by clicking View Part for each listed below this table.

Query Start/End: The start/end index of the part on the insert sequence. Defaults to 0.
Subject Start/End: The start/end index of the part on its original sequence. Defaults to 0.
Position: The location of the part in the assembly solution.
Name Database Length Extended Length Query Start Query End Subject Start Subject End Position
BBa_K165060-igem igem 195 218 4 199 2456 2237 0
55047.2-addgene addgene 707 750 203 910 7972 8682 1
BBa_S05052-igem igem 35 57 914 949 81 137 2
99831-addgene addgene 704 744 953 1657 4786 5502 3
pGGAselect NONE 2143 2174 1702 3849 0 0 4

Plasmid Map

Parts List

BBa_K165060-igem igem View Part

218nt Extended 195nt Template

  • Assembly Sequence: GGTCTCNGGTACGGAGgaagagcgc...tgtggaattgtgagcggaNGAGACC
  • Template Sequence: gaagagcgcccaatacgcaaaccgc...tatgttgtgtggaattgtgagcgga

55047.2-addgene addgene View Part

750nt Extended 707nt Template

  • Assembly Sequence: GGTCTCNcggataacaatttcacac...catggacgagctgtacaaNGAGACC
  • Template Sequence: atggtgagcaagggcgaggaggata...ccggcggcatggacgagctgtacaa

BBa_S05052-igem igem View Part

57nt Extended 35nt Template

  • Assembly Sequence: GGTCTCNacaagtaaggtgatacca...cttgatgcccttggcagcNGAGACC
  • Template Sequence: ggtgataccagcatcgtcttgatgc...gcatcgtcttgatgcccttggcagc

99831-addgene addgene View Part

744nt Extended 704nt Template

  • Assembly Sequence: GGTCTCNcagcaccctgctaaggta...tacacatggcatggatgaNGAGACC
  • Template Sequence: atgagtaaaggagaagaacttttca...ctgggattacacatggcatggatga

pGGAselect View Part

2174nt Extended 2143nt Template Internal Cutsite(s) - Adjust probability of success for future experiments

  • Assembly Sequence: GGTCTCNatgagctctacaaataaC...GAATTCGAAGACTTGGTANGAGACC
  • Template Sequence: CCATGGTCTCACCATTCCTGTAGTC...GGGATCCGAATTCGAAGACTTGGTA

Primers Download Primers

Below is a summary table of the primers needed for each part of this assembly solution (shown above). Further details on each primer can be viewed by clicking View Primer for each listed below this table.

hp Tm (C): The melting temperature of the primer's hairpin (hp) structure.
hp ΔG: The Gibbs free energy of the primer's hairpin (hp) structure.
hd Tm (C): The melting temperature of the primer's homodimer (hd) structure.
hd ΔG: The Gibbs free energy of the primer's homodimer (hd) structure.
Name Type Tm (C) GC% Hairpin hp Tm (C) hp ΔG Homodimer hd Tm (C) hd ΔG
BBa_K165060-igem forward primer fwd 60.181 28.12 False 0.000 0.000 True 2.126 -5818.851
BBa_K165060-igem reverse primer rvs 60.369 34.78 False 0.000 0.000 True -41.284 -1762.190
55047.2-addgene forward primer fwd 62.012 41.18 True 40.527 -577.899 True 3.513 -3469.324
55047.2-addgene reverse primer rvs 61.541 36.0 False 0.000 0.000 True -8.830 -3413.286
BBa_S05052-igem forward primer fwd 59.473 35.48 True 41.613 -271.143 True 0.084 -3163.304
BBa_S05052-igem reverse primer rvs 61.930 45.0 True 58.011 -1529.043 True 9.633 -5021.834
99831-addgene forward primer fwd 60.173 35.71 True 54.635 -1135.184 True -5.970 -2788.364
99831-addgene reverse primer rvs 60.961 33.33 False 0.000 0.000 True 3.340 -4081.791
pGGAselect forward primer fwd 59.878 12.2 True 45.846 -1480.848 True 19.742 -7809.981
pGGAselect reverse primer rvs 59.398 0.0 True 48.367 -876.789 True 21.497 -7616.761

Primers List

BBa_K165060-igem forward primer View Primer

fwd 60.181°C Homodimer

  • Sequence: GGTCTCNGGTACGGAGgaagagcgcccaatac

BBa_K165060-igem reverse primer View Primer

rvs 60.369°C Homodimer

  • Sequence: GGTCTCNtccgctcacaattcca

55047.2-addgene forward primer View Primer

fwd 62.012°C Hairpin Homodimer

  • Sequence: GGTCTCNcggataacaatttcacacaggaaacagctatggtgagcaagggc

55047.2-addgene reverse primer View Primer

rvs 61.541°C Homodimer

  • Sequence: GGTCTCNttgtacagctcgtccatg

BBa_S05052-igem forward primer View Primer

fwd 59.473°C Hairpin Homodimer

  • Sequence: GGTCTCNacaagtaaggtgataccagcatcg

BBa_S05052-igem reverse primer View Primer

rvs 61.930°C Hairpin Homodimer

  • Sequence: GGTCTCNgctgccaagggca

99831-addgene forward primer View Primer

fwd 60.173°C Hairpin Homodimer

  • Sequence: GGTCTCNcagcaccctgctaaggtaacaacaagatgagtaaaggagaagaactttt

99831-addgene reverse primer View Primer

rvs 60.961°C Homodimer

  • Sequence: GGTCTCNtcatccatgccatgtgt

pGGAselect forward primer View Primer

fwd 59.878°C Hairpin Homodimer

  • Sequence: GGTCTCNatgagctctacaaataaCCATGGTCTCACCATTC

pGGAselect reverse primer View Primer

rvs 59.398°C Hairpin Homodimer

  • Sequence: GGTCTCNTACCAAGTCTTCGAATTCG
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