CS2 15-nt Tail Solution PCR

July 24, 2025, 6:38 p.m.

  • 4 Parts 8 Primers
  • 60.78C Average Primer Tm 26nt Average Primer 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
  • 80.00% BLAST nt 20.00% Synthetic nt
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Parts Download Parts

Below is a summary table of the DNA sequences that make up this assembly solution. Further details on each part can be viewed by clicking View Part for each listed below this table.

Query Start/End: The start/end index of the part on the insert sequence. Defaults to 0.
Subject Start/End: The start/end index of the part on its original sequence. Defaults to 0.
Position: The location of the part in the assembly solution.
Name Database Length Extended Length Query Start Query End Subject Start Subject End Position
120397-addgene addgene 133 147 0 133 8436 8304 0
46918.2-addgene addgene 711 725 133 844 124 834 1
Synthetic-1.112 NONE 112 126 844 956 0 0 2
73974-addgene addgene 717 731 956 1673 19309 20025 3
pBR322_b NONE 4361 4375 1674 6034 0 0 4

Plasmid Map

Parts List

120397-addgene addgene View Part

147nt Extended 133nt Template

  • Assembly Sequence: TTGACAGgtgagctgataccgctcg...taatgcagctggcacgacatggtga
  • Template Sequence: gtgagctgataccgctcgccgcagc...gattcattaatgcagctggcacgac

46918.2-addgene addgene View Part

725nt Extended 711nt Template

  • Assembly Sequence: gcacgacatggtgagcaagggcgag...gacgagctgtacaagtaaggtgata
  • Template Sequence: atggtgagcaagggcgaggaggata...cggcatggacgagctgtacaagtaa

Synthetic-1.112 View Part

126nt Extended 112nt Template

  • Assembly Sequence: caagtaaggtgataccagcatcgtc...tcccaattcttgttgaatatgagta
  • Template Sequence: ggtgataccagcatcgtcttgatgc...ggagttgtcccaattcttgttgaat

73974-addgene addgene View Part

731nt Extended 717nt Template

  • Assembly Sequence: gttgaatatgagtaaaggagaagaa...gatgaactatacaaataaCTTATCA
  • Template Sequence: atgagtaaaggagaagaacttttca...tggcatggatgaactatacaaataa

pBR322_b View Part

4375nt Extended 4361nt Template

  • Assembly Sequence: caaataaCTTATCATCGATAAGCTT...AATTCTCATGTTTGACAGgtgagct
  • Template Sequence: CTTATCATCGATAAGCTTTAATGCG...CTTCAAGAATTCTCATGTTTGACAG

Primers Download Primers

Below is a summary table of the primers needed for each part of this assembly solution (shown above). Further details on each primer can be viewed by clicking View Primer for each listed below this table.

hp Tm (C): The melting temperature of the primer's hairpin (hp) structure.
hp ΔG: The Gibbs free energy of the primer's hairpin (hp) structure.
hd Tm (C): The melting temperature of the primer's homodimer (hd) structure.
hd ΔG: The Gibbs free energy of the primer's homodimer (hd) structure.
Name Type Tm (C) GC% Hairpin hp Tm (C) hp ΔG Homodimer hd Tm (C) hd ΔG
120397-addgene forward primer fwd 60.471 39.13 True 41.615 -385.626 True 19.277 -7324.651
120397-addgene reverse primer rvs 61.684 61.9 True 41.769 -271.074 True 8.420 -5998.441
46918.2-addgene forward primer fwd 62.012 63.64 True 40.104 -225.942 True 6.006 -3199.547
46918.2-addgene reverse primer rvs 61.920 46.15 False 0.000 0.000 True -8.830 -3413.286
Synthetic-1.112 forward primer fwd 59.473 47.83 True 41.613 -271.143 True 0.084 -3163.304
Synthetic-1.112 reverse primer rvs 60.130 33.33 True 53.877 -1047.648 True -23.066 -2929.371
73974-addgene forward primer fwd 60.173 30.0 True 37.663 -70.162 True -20.930 -3085.011
73974-addgene reverse primer rvs 60.214 23.33 False 0.000 0.000 True -5.269 -2789.681
pBR322_b forward primer fwd 60.806 3.23 True 48.641 -1501.254 True 18.725 -5292.685
pBR322_b reverse primer rvs 60.872 12.9 True 36.900 11.873 True 9.560 -5625.021

Primers List

120397-addgene forward primer View Primer

fwd 60.471°C Hairpin Homodimer

  • Sequence: TTGACAGgtgagctgataccgct

120397-addgene reverse primer View Primer

rvs 61.684°C Hairpin Homodimer

  • Sequence: tcaccatgtcgtgccagctgc

46918.2-addgene forward primer View Primer

fwd 62.012°C Hairpin Homodimer

  • Sequence: gcacgacatggtgagcaagggc

46918.2-addgene reverse primer View Primer

rvs 61.920°C Homodimer

  • Sequence: tatcaccttacttgtacagctcgtcc

Synthetic-1.112 forward primer View Primer

fwd 59.473°C Hairpin Homodimer

  • Sequence: caagtaaggtgataccagcatcg

Synthetic-1.112 reverse primer View Primer

rvs 60.130°C Hairpin Homodimer

  • Sequence: tactcatattcaacaagaattgggaca

73974-addgene forward primer View Primer

fwd 60.173°C Hairpin Homodimer

  • Sequence: gttgaatatgagtaaaggagaagaactttt

73974-addgene reverse primer View Primer

rvs 60.214°C Homodimer

  • Sequence: TGATAAGttatttgtatagttcatccatgc

pBR322_b forward primer View Primer

fwd 60.806°C Hairpin Homodimer

  • Sequence: caaataaCTTATCATCGATAAGCTTTAATGC

pBR322_b reverse primer View Primer

rvs 60.872°C Hairpin Homodimer

  • Sequence: agctcacCTGTCAAACATGAGAATTCTTGAA
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