CS2 15-nt Tail Golden Gate

July 24, 2025

This is an overview of your assembly. Under the Solutions section you will find solutions that Smithy has created for you. Click on View Solution to see the parts (purchasable DNA sequences), primer designs for each part, and more that Smithy has created for that unique assembly solution.

The cost and time calculations exclude the cost and time of experimental steps that are common to all compared cloning methods.

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General Info

  • Method: Golden Gate
  • Overhangs: 15 overhangs - 98.5% fidelity

BLAST Query

  • AddGene iGEM
  • Min BLAST seq size: 100nt
  • Max BLAST seq size: 1000nt
  • Min synthetic seq size: 50nt
  • Max synthetic seq size: 200nt

Experiment

  • Monovalent ion concentration: 50.0mM
  • Divalent ion concentration: 1.5mM
  • dNTP concentration: 0.8mM
  • DNA concentration: 50.0nM
  • Melting temperature: 60.0C

Solutions

CS2 15-nt Tail Solution View Solution

July 24, 2025, 6:38 p.m.
  • 4 Parts 8 Primers
  • 60.52C Average Primer Tm 33nt Average Primer
  • 81.00% BLAST nt 19.00% Synthetic nt