CS2 15-nt Tail Bundle
July 24, 2025, 6:38 p.m.
Case Study 2, bundle assembly with 15nt tail
These cost and time calculations exclude the cost and time of experimental steps that are common to all compared cloning methods.
Gibson
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CS2 15-nt Tail Solution
A Gibson solution
- 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
- 60.78C Average Primer Tm 26nt Average Primer
- 80.00% BLAST nt 20.00% Synthetic nt
Golden Gate
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CS2 15-nt Tail Solution
A Golden Gate solution
- 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 700nt Longest Part 111nt Shortest Part
- 60.52C Average Primer Tm 33nt Average Primer Restriction Enzymes
- 81.00% BLAST nt 19.00% Synthetic nt
PCR
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CS2 15-nt Tail Solution
A PCR solution
- 4 Parts 8 Primers 3 BLAST Parts 1 Synthetic Parts 717nt Longest Part 112nt Shortest Part
- 60.78C Average Primer Tm 26nt Average Primer
- 80.00% BLAST nt 20.00% Synthetic nt